Characterisation of Novel Seed Endophytes in Medicago sativa

Dublin Core

Title

Characterisation of Novel Seed Endophytes in Medicago sativa

Abstract

The microbiome of plants is essential in their development and health. Seed endophytes are microorganisms that live within the seeds of plants, the majority of which are believed to be non-pathogenic, although more research is needed to better understand the function of seed endophytes. They are present from the beginning growth stages and play many important roles. A variety of bacterial species have been found as seed endophytes. They have also been found to have common functions for colonization, benefiting plant growth, and antifungal properties. In this project, seed endophytes were isolated from surface sterilized Medicago sativa seeds on selective media for potassium solubilization, actinomycetes, and nitrogen free media. Sterilized seeds were germinated and planted in Yosida agar. The sterilized plants were then inoculated with the seed endophyte isolates and uptake of the isolates into the plant roots was measured. The methods were improved, germinating the seeds in sterile water often led to self toxicity and the seeds germinated at a much higher rate using the Murashige and Skoog Medium. Several sources of contamination within the protocol were found and eliminated. The isolates also underwent DNA extraction and colony PCR for 16S rRNA gene sequencing. Using this method a Pseudomonas species was identified. Seed endophytes from Medicago sativa were characterized.

Creator

Sarah Lott

Date

5/1/2022

Contributor

Katherine Moccia
Microbiology

Rights

U.S. and international copyright laws may protect this work. It is provided by Wagner College for scholarly or research purposes only. Commercial use or distribution is not permitted without prior permission of the copyright holder.

Format

application/pdf

Extent

50 pages

Language

eng

Type

text

Identifier

2022_Microbiology_Lott - Sara Guariglia

Rights Holder

Wagner College, Staten Island, NY

Document Item Type Metadata

Original Format

Thesis

Date Digital

2022

Citation

Sarah Lott, “Characterisation of Novel Seed Endophytes in Medicago sativa,” Wagner College Archives and Special Collections, accessed November 30, 2022, https://wagnercollections.omeka.net/items/show/19409.